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2011年秋季先进机器人与MEMS技术系列学术讲座（42）
添加日期：2011-11-28 作者：李昭老师 来源：
南开大学机器人与信息自动化研究所
Institute of Robotics and Automatic Information System 2011年秋季先进机器人与MEMS技术系列学术讲座 Seminar Series：Advanced Robotics & MEMS 题目：Dynein achieves processivity using both stochastic and tension coordinated stepping 地点：三教301多媒体教室 时间：11月30日（本周三）下午14：30~16:00 报告人：仇伟红 博士 Seminar Speaker: Dr. Weihong Qiu 单位： 哈佛大学医学院细胞生物系 Seminar Speaker Affiliation: Department of Cell Biology, Harvard Medical School 讲座摘要： Processivity, the ability of single molecules to move continuously along a track, is a fundamental requirement of molecular motors that transport cargo long distances. Here, we investigate how cytoplasmic dynein, a homodimeric, microtubule-based motor, achieves processive motion. To analyze how each motor domain of the dynein dimer moves, we developed a versatile method for assembling dynein heterodimers using complementary DNA oligonucleotides covalently linked to dynein monomers labeled with different organic fluorophores. Using two-color, single-molecule microscopy and high-precision, two-dimensional tracking, we find that the motor domains show a highly variable stepping pattern that is distinct from all processive dimeric myosin and kinesin motors, which use a “hand-over-hand” mechanism. Uniquely, dynein stepping is stochastic when the two motor domains are close together. However, patterns emerge as the distance between motor domains increases, implying a tension-based mechanism governs these steps. The plasticity of dynein’s stepping mechanism may allow tuning of dynein for the many cellular functions it performs. 主讲人简历 ： 仇伟红博士，哈佛大学医学院细胞生物系博士后。于1999年和2002年在南开大学物理学院获理学学士和硕士学位，2008年在美国俄亥俄州立大学哥伦布分校获理学博士。仇伟红博士目前在哈佛医学院的研究课题是用单分子生物物理方法去揭示动力蛋白分子机制和基于动力蛋白的胞内运输的调控机理，在Nature Structural & Molecular Biology, Molecular Cell, PNAS 等著名刊物上发表论文十余篇。 |